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Dr.
John J. Wyrick
.jpg) 509-335-8785
jwyrick@wsu.edu
Assistant
Professor, School of Molecular Biosciences. Ph.D. 2002, M.I.T.
Research
Cells respond
to environmental or developmental signals by reprogramming the expression
of specific genes throughout their genome. We wish to understand
the mechanisms by which this occurs. To do so, we have used genomics-based
approaches to decipher how gene expression is regulated in the model
eukaryote, Saccharomyces cerevisiae, and in the plant, Arabidopsis
thaliana. Ongoing projects include the (1) use of DNA microarrays
to profile genome-wide mRNA levels in various transcription factor
and histone mutant strains; (2) analysis of global protein-DNA interactions
using DNA microarrays; (3) the development of various bioinformatics
tools to analyze the microarray data, including an online microarray
database. These projects involve the development and use of functional
genomic tools to investigate how genome expression is regulated
in eukaryotic cells.
Selected
Publications
Parra, M.A., Kerr, D., Fahy, D., Pouchnik, D.J., and Wyrick, J.J.
2006. Deciphering the Roles of the Histone H2B N-terminal domain
in Genome-wide Transcription. Mol. Cell Biol. 26:3842-3852.
O'Connor, T.R., Dyreson, C., and Wyrick, J.J. 2005. Athena: a
resource for rapid visualization and systematic analysis of Arabidopsis
promoter sequences. Bioinformatics 21:4411-4413.
Martin, A.M.,
Pouchnik, D.J., Walker, J.L., and Wyrick, J.J. 2004.
Redundant Roles for Histone H3 N-Terminal Lysine Residues in Subtelomeric
Gene Repression in Saccharomyces cerevisiae. Genetics 167:1123-1132.
Lee, T.I., Rinaldi,
N.J., Robert, F., Odom, D.T., Bar-Joseph, Z., Gerger, G.K., Hannett,
N.M., Harbison, C.T., Thompson, C.M., Simon, I., Zeitlinger,
J., Jennings, E.G., Murray, H.L., Gordon, D.B., Ren, B., Wyrick,
J.J., Tagne, J.B., Volkert, T.L., Fraenkel, E., Gifford, D.K.
and Young, R.A. 2002. Transcriptional Regulatory
Networks in Saccharomyces cerevisiae. Science 298:
799-804.
Wyrick, J.J.
and Young, R.A. 2002. Deciphering Gene Expression
Regulatory Networks. Curr. Opin. Genet. Dev. 12:130-6.
Simon, I., Barnett,
J., Hannett, N., Harbison, C.T., Rinaldi, N.J., Volkert, T.L.,
Wyrick, J.J., Zeitlinger, J., Gifford, D.K., Jaakkola, T.S.,
and Young, R.A. 2001. Serial Regulation of
Transcriptional Regulators in the Yeast Cell Cycle. Cell 106:
697-708.
Wyrick, J.J.,
Aparicio, J.G., Chen, T., Barnett, J.D., Jennings, E.G., Young,
R.A., Bell, S.P. and Aparicio, O.M. 2001. Genome-Wide
Distribution of ORC and MCM proteins in S. cerevisiae:
High-Resolution Mapping of Replication Origins. Science 294:2357-2360.
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