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Dr. Clarence A. Ryan
.jpg) In
Memorium
September 29, 1931-
October 7, 2007
A
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Research
The major goals
of this program are to investigate the regulation of synthesis of
defensive proteins in plants that are induced by predator and pathogen
attacks. Nearly 20 proteins have been identified whose systemic
synthesis is regulated in response to herbivore and pathogen attacks.
A lipid-based signal transduction pathway has been found to mediate
the response. Signals from the plants have been identified and characterized,
including a novel polypeptide signal, called systemin, analogous
to polypeptide signals in animals, oligouronide fragments from the
plant cell wall, and jasmonic acid, a prostagandin analog. Elucidating
the biochemistry and cell biology of this pathway is the focus of
our present research. The application of this knowledge to improve
crop production and to understand ecological systems is a major
goal of the program.
Selected
Publications
Huffaker, A., and C.A. Ryan. 2007. Endogenous
peptide defense signals in Arabidopsis differentially amplify signaling
for the innate immune response. Proc. Natl. Acad. Sci. USA. In
press.
Pearce, G., W.F. Siems, R. Bhattacharya, Y.-C.
Chen, and C.A. Ryan. 2007. Three hydroxyproline-rich glycopeptides
derived from a single petunia polyprotein precursor activate Defensin
I, a pathogen defense response gene. J. Biol. Chem. In press (online).
Pearce, G., J. Narváez-Vásquez, and
C.A. Ryan. 2006. Systemins, in Handbook of Bioactive Peptides, Abba
Kastan ed., pp. 49-53.
Huffaker, A., Y. Yamaguchi, G. Pearce., and C.A.
Ryan. 2006. AtPep1, in Handbook of Bioactive
Peptides, Abba Kastan ed., pp. 5-8.
Moura, D.S., G. Pearce, and C.A.
Ryan. 2006. RALF, in Handbook of Bioactive Peptides,
Abba Kastan ed., pp. 33-36.
Srintanyarat, W., G. Pearce, W.F. Siems, C.A. Ryan,
R. Wititsuwannakul, and G. Wititsuwannakul. 2006. Isolation and
characterization of isoinhibitors of the potato inhibitor I family
from the latex of rubber trees, Hevea brasiliensis. Phytochemistry
67:1644-1650.
Yamaguchi, Y., G. Pearce, W.F. Siems, and C. A.
Ryan. 2006. The cell surface leucine-rich repeat receptor for AtPep1,
an endogenous elicitor in Arabidopsis, is functional in transgenic
tobacco cells. Proc. Natl. Acad. Sci. USA. 103:10104-10109.
Huffaker, A., G. Pearce, and C.A. Ryan. 2006. AtPep1,
An endogeous pepide signal in Arabidopsis activates components
of the innate immune response. Proc. Natl. Acad. Sci. USA 103:10098-10103.
Yamaguchi, Y., G. Pearce, and C.A. Ryan.
2006. The cell surface leucine-rich repeat receptor for AtPep1,
an endogenous elicitor in Arabidopsis, is functional in transgenic
tobacco cells. Proc. Natl. Acad. Sci. USA 103:10104-10109.
Ryan, C. A., G. Pearce, and J. Narváez-Vásquez.
Cell Wall-Associated Peptides of the Systemin Family of Defense
Signals. 2006. In The Science and Lore of the Plant Cell
Wall, T. Hayashi, ed. Brown Walker Press. pp. 31-316.
Scheer, J., G. Pearce, and C.A. Ryan. 2005.
LeRALF, a plant peptide that regulates root growth and development,
specifically binds to 25 kD and 120 kD cell surface membrane proteins
of Lycopersicon
peruvianum. Planta. 221:667 674.
Narváez-Vásquez, J., G. Pearce,
and C.A. Ryan. 2005. The plant cell wall matrix harbors a
precursor of defense signaling peptides. Proc. Natl. Acad. Sci.
USA. 102(36):12974-12977.
Narváez-Vásquez, J. and C.A. Ryan.
2004. The cellular localization of prosystemin: A functional role for
phloem parenchyma in systemin signaling. Planta 218:360-369.
Ryan, C. A. and G. Pearce. 2004. Peptide
hormones/systemins. In Encyclopedia of
Biological Chemistry, W. Lennarz and M. D. Lane Eds. Elsevier.
Volume 3, 381-384.
Diez-Diaz, M., V. Conejero, I. Rodrigo, G. Pearce,
and C.A. Ryan. 2004. Isolation and characterization of wound-inducible
carboxypeptidase inhibitor from tomato leaves. Phytochemistry 65:1919-1924.
Ryan, C. A. and G. Pearce. 2004. Peptide hormones
for defense, growth, and development. In Plant Hormones,
P. J. Davies ed. Elsevier, pp. 654-670.
Orozco-Cardenas, M. and C.A. Ryan. 2002.
Nitric oxide inhibits defense gene expression in leaves of wounded
tomato plants. Plant Physiol. in press.
Ryan, C.A. and D.S. Moura. 2002. Systemic wound
signaling in plants: A new perception. Proc. Natl. Acad. Sci. USA. 99:6519-6520.
Ryan, C.A. and G. Pearce. 2002. Polypeptide
hormones in plants. In Encyclopedia of Plant Pathology.
Ryan, C.A., G. Pearce, D. Moura, and J.M. Scheer.
2002. Polypeptide hormones. Plant Cell 14:251-264.
Scheer, J.M. and C.A. Ryan. 2002. The systemin
receptor SR160 from Lycopersicon peruvianum is a member of the
LRR receptor kinase family. Proc. Natl. Acad. Sci. USA. 99:9585-9590.
Pearce, G., D.S. Moura, J. Stratmann, and
C.A. Ryan. 2001. RALF, a 49 amino acid polypeptide signal
arrests root growth and development. Proc. Natl. Acad. Sci. USA 98:12843-12847.
Scheer, J.M. and C.A. Ryan. 2001. A method
for the quantitative recovery of proteins from polyacrylamide gels.
Analytical Biochemistry
238:130-132.
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