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Dr.
B.W. Poovaiah
.jpg) 509-335-2487
poovaiah@wsu.edu
Regents Professor,
Department of Horticulture, Ph.D.1970, Utah State University.
Poovaiah lab website:
http://molecularplants.wsu.edu/calcium/
Research
This
laboratory has a long-standing interest in understanding the Ca2+/calmodulin-mediated
signaling in plants. Calmodulin (CaM), a ubiquitous and multifunctional
Ca2+-binding protein, is a primary intracellular Ca2+ receptor,
which transduces the second messenger Ca2+ signal by binding to
and altering the activity of the other proteins. To study the downstream
elements involved in Ca2+/CaM-mediated stress signal transduction,
35S-labeled CaM was used for protein-protein interaction-based
screening of several expression libraries. By this approach, we
have cloned and characterized several genes that encode for CaM-binding
proteins. The primary goal of this research is to investigate the
functional significance of these genes, and understand how they
are involved in Ca2+/CaM-mediated signaling. Ca2+/CaM-regulated
protein phosphorylation is believed to play a pivotal role in amplifying
and diversifying the action of Ca2+-mediated signals. This laboratory
has cloned a chimeric Ca2+/CaM-dependent protein kinase (CCaMK)
gene. CCaMK is characterized by the presence of a kinase domain,
an autoinhibitory domain, a CaM-binding domain and a neural visinin-like
Ca2+-binding domain in a single polypeptide. This laboratory has
also cloned a Ca2+/CaM-dependent receptor-like kinase, and the
role of this and other CaM-binding proteins are being investigated.
Image
Selected
Publications
U.S. patents: No. 5, 498, 533 (1996); No. 6, 077, 991 (2000);
No. 6, 362, 395 (2002) and No. 6, 403, 352 (2002).
Gleason, C., Chaudhuri, S., Yang, T., Munoz-Gutierrez, A., Poovaiah,
B.W. and Oldroyd, G.E.D. Nodulation independent of rhizobia induced
by a calcium-activated kinase lacking autoinhibition, Nature (in
press).
Du, L. and Poovaiah, B. W. 2005. Ca2+/calmodulin-binding is critical
for DWARF1 function in brassinosteroid biosynthesis and plant growth
and development, Nature 437:741-745.
Du, L. and Poovaiah, B. W. 2004. A
novel family of Ca2+/calmodulin-binding proteins involved in transcriptional
regulation: Interaction
with fsh/Ring3 class transcription activators. Plant Mol. Biol.
54:549-569.
Yang, T., Chaudhuri, S., Yang, L., Chen,
Y., and Poovaiah, B. W. 2004. Calcium/calmodulin
upregulates a cytoplasmic receptor-like kinase. J. Biol. Chem.
279:42552-42559.
Yang, T. and Poovaiah, B.W. 2003. Calcium/calmodulin-mediated
signal network in plants. Trends Plant Sci. 8:505-512.
Sathyanarayanan, P.V. and Poovaiah, B.W. 2002. Autophosphorylation-dependent
inactivation of plant chimeric calcium/calmodulin-dependent protein
kinase. Eur. J. Biochem. 269:2457-2463.
Yang, T. and Poovaiah, B.W. 2002. A calmodulin-binding/CGCG
box DNA-binding protein family involved in multiple signal pathways
in plants. J. Biol. Chem. J. Biol. Chem. 277:45049-45058.
Yang, T. and Poovaiah, B.W. 2002. Hydrogen peroxide homeostasis:
Activation of plant catalase by calcium/calmodulin. Proc. Natl.
Acad. Sci. 99:4097-4102.
Sathyanarayanan, P.V., Siems, W.F., Jones, J.P. and Poovaiah,
B.W. 2001. Calcium-stimulated autophosphorylation site of plant
chimeric calcium/calmodulin dependent protein kinase. J. Biol.
Chem. 276:32940-32947.
Sathyanarayanan, P.V., Cremo, C.R. and Poovaiah, B.W. 2000. Plant
chimeric Ca2+/calmodulin-dependent protein kinase: Role of the
neural visinin-like domain in regulating autophosphorylation and
calmodulin affinity. J. Biol. Chem. 39:30417-30422.
Yang, T. and Poovaiah, B.W. 2000. An early ethylene up-regulated
gene encoding a calmodulin-binding protein involved in plant senescence
and death. J. Biol. Chem. 275:38467-38473.
Yang, T. and Poovaiah, B.W. 2000. Molecular and biochemical evidence
for the involvement of calcium/calmodulin in auxin action. J. Biol.
Chem. 275:3137-3143.
Poovaiah, B.W., Xia, M., Liu, Z., Wang, W., Yang, T., Sathyanarayanan,
P.V. and Franceschi, V.R. 1999. Developmental regulation of the
gene for chimeric calcium/calmodulin-dependent protein kinase gene
in anthers. Planta 209:161-171.
Wang, W. and Poovaiah, B.W. 1999. Interaction
of plant chimeric calcium/calmodulin‑dependent protein kinase with a homolog
of eukaryotic elongation factor‑1 alpha. J. Biol. Chem. 274:12001-12008.
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